Cdc42 takes a role in the chemotaxis of umbilical cord mesenchymal stem cells to inflammatory cytokines

doi:10.3969/j.issn.2095-4344.2014.23.002

Abstract

Abstract:

BACKGROUND: The homing ability of mesenchymal stem cells is closely associated with the effects of cell transplantation. Clarifying the mechanism of chemotaxis and migration will contribute to enhance the clinical application of mesenchymal stem cells.

OBJECTIVE: To investigate the effect of Cdc42 in the homing of human umbilical cord mesenchymal stem cells.

METHODS: First, mesenchymal stem cells were isolated from human umbilical cord, and co-cultured with tumor necrosis factor α, interleukin-1β, and transforming growth factor β. Western blot assay was used to test the level of Cdc42. Besides, Cdc42 siRNA was synthesized by chemical method to transfect the cells, and cell migration and adhesion were measured by Transwell and Matrigel separately. Meanwhile, the activity of signal molecule, extracellular regulated protein kinase 1/2, was evaluated by western blot.

RESULTS AND CONCLUSION: The results indicated that the inflammation factors induced the highly expression of Cdc42 in human umbilical cord mesenchymal stem cells, almost double level to controls. siRNA notably inhibited the migration and adhesion of human umbilical cord mesenchymal stem cells through Cdc42 down-regulation, and the extracellular regulated protein kinase 1/2 and phosphorylation form were also decreased simultaneously. In a word, we speculate Cdc42 plays a role in the chemotaxis of human umbilical cord mesenchymal stem cells in vitro.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, cdc42 GTP-binding protein, cell movement

CLC Number:

R394.2

Liu Su-rui, Li Jun-xia, Yang Xiao-ya, Li Zhu, Gao Yu-hua, Xu Sheng-ru, Wang Geng-yin. Cdc42 takes a role in the chemotaxis of umbilical cord mesenchymal stem cells to inflammatory cytokines[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(23): 3616-3621.

Figures/Tables 3

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